Human Anti-IGF1R Recombinant Antibody (TAB-199)

Name: Human Anti-IGF1R Recombinant Antibody (TAB-199)
SKU: TAB-199
Rating: 5 (1 reviews)

CAT#: TAB-199

This product is a recombinant Human antibody that recognizes IGF1R. The antibody was purified by affinity chromatography.

Tested Data Gene Expression

ELISA

Figure 1 Anti-Human IGF1R Antibody (TAB-199) in ELISA

ELISA analysis of TAB-199 was performed by coating with human IGF1R protein.

The secondary antibody: HRP-Anti-Human IgG (H+L)

Figure 2 Anti-Human IGF1R Antibody (TAB-199) in WB

Western blot analysis of TAB-199 was performed with human IGF1R protein.

TAB-199 incubation concentration: 2ng/μL.
The secondary antibody: HRP-Anti-Human IgG (H+L)
Lane 1: Reducing antigen (0.1μg)
Lane 2: Reducing antigen (0.3μg)
Lane 3: Reducing antigen (0.6μg)

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Normal Tissue Figure 1 Cerebral cortex

Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Neuropil
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10268/32662_B_7_5.jpg

Normal Tissue Figure 2 Colon

Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10268/32662_A_7_3.jpg

Normal Tissue Figure 3 Liver

Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10268/32662_A_8_4.jpg

Normal Tissue Figure 4 Kidney

Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10268/32662_A_7_5.jpg

Normal Tissue Figure 5 Testis

Cells in seminiferous ducts
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10268/32662_A_6_6.jpg

Normal Tissue Figure 6 Heart muscle

Cardiomyocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10268/32662_B_5_6.jpg

Normal Tissue Figure 7 Lymph node

Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10268/32662_A_7_8.jpg

RNA Expression Figure 8 RNA cell line category: Low cell line specificity

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000140443-IGF1R

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Specifications

Immunogen
The details of the immunogen for this antibody are not available.

Host Species
Human

Derivation
Human

Type
Human IgG1, κ

Species Reactivity
Human

Applications
FC, IP, ELISA, Neut, FuncS, IF, ICC, Inhib, WB, Activ, IHC, Block

Related Disease
Cancers

Product Property

Purity
>95% as determined by SDS-PAGE

Concentration
Please refer to the vial label for the specific concentration

Buffer
PBS

Preservative
No preservatives

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
The IGF1 antibody has been reported in applications of FC, IP, ELISA, Neut, FuncS, IF, ICC, Inhib, WB, Activ, IHC, Block.

Target

Alternative Names
IGFR; CD221; IGFIR; JTK13

Gene ID
3480

UniProt ID
P08069

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To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# TAB-199, RRID: AB_3111868)

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Related Signaling Pathways

Insulin Signaling Pathway

Related Diseases

Endocrine Resistance

Type I Diabetes Mellitus

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - TAB-199.pdf

MSDS

MSDS - TAB-199.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunofluorescence (IF)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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